{"id":1151,"date":"2018-09-06T09:48:05","date_gmt":"2018-09-06T09:48:05","guid":{"rendered":"http:\/\/priorclavena.com\/?p=1151"},"modified":"2025-01-02T16:49:51","modified_gmt":"2025-01-02T16:49:51","slug":"top-tips-for-autoclaving-pipette-tips","status":"publish","type":"post","link":"https:\/\/www.priorclave.com\/en-us\/2018\/09\/06\/top-tips-for-autoclaving-pipette-tips\/","title":{"rendered":"Top Tips for Autoclaving Pipette Tips"},"content":{"rendered":"

You don’t have to spend much time in a research lab to learn that pipette tips have a nasty habit of stubbornly clinging to a little bead of water.\u00a0 <\/span>Some labs go so far as wrapping everything in foil in an attempt to “keep the moisture out.”<\/p>\n

\ud83d\ude27<\/span> Terrible practice!<\/i><\/p>\n

First, this is going to trap moisture in the box, assuring you wind up with wet tips. Second, steam autoclaves<\/a> only work when the steam can circulate freely to heat the items.\u00a0 <\/span>If the moist steam hasn’t reached the tips, then the dips are not sterile.\u00a0 <\/span>Wrapping your tip boxes in an attempt to keep the tips dry will result in 1) damp tips that 2) are not sterile!<\/i><\/p>\n

How to Autoclave Dry(er) Pipette Tips<\/h2>\n

Nine times out of ten, a few minor<\/i> changes in your standard lab practice will minimize (or even eliminate) damp tips.<\/p>\n

Four things to keep in mind:<\/p>\n

    \n
  1. Don’t bother autoclaving sterile tips!<\/b>\u00a0 <\/span>If they come sterile and in a sealed pack, they are sterile; don’t beat a sterile horse.<\/li>\n
  2. Use the autoclave’s dry setting<\/b>. The dry cycle is very bad for liquids (the lower chamber pressure will boil off\u2014and even boil over\u2014some of your liquid volume), but great for producing (nearly) dry loads.<\/li>\n
  3. Make sure you have good air circulation<\/b>. Don’t try to keep the tips dry; embrace the purifying steam!<\/li>\n
  4. Give them time<\/b>! If you just let them sit in the autoclave to cool off, tips will dry on their own.\u00a0 <\/span>You can speed this process along by transferring your tip boxes to a heated drying cabinet (or even a spare incubator set at 55\u00baC).<\/li>\n<\/ol>\n

    The Best Recipe for Sterilizing Pipette Tips<\/h2>\n

    Provided your tips actually need to be autoclaved<\/a> (remember<\/b>: Never waste time sterilizing sterile packaged items), then this is a good, reliable method:<\/p>\n

      \n
    1. Open the lid of the tip tray a tad<\/b>\u2014propping it up with the lid’s locking tab helps; a little tape can hold it in place\u2014don’t wrap the box in foil<\/a>!<\/i><\/li>\n
    2. Run your dry cycle<\/i> as normal<\/b><\/li>\n
    3. Let the damp tips dry in the warm autoclave<\/b> (or transfer to a drying cabinet\/incubator)<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

      You don’t have to spend much time in a research lab to learn that pipette tips have a nasty habit of stubbornly clinging to a little bead of water.\u00a0 Some […]<\/p>\n","protected":false},"author":15,"featured_media":3516,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[73],"tags":[72,74,75,76,77,78,79,80],"class_list":["post-1151","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lab-autoclaves","tag-autoclaving-tips","tag-lab-practices","tag-lab-work","tag-pipette-tips","tag-research-lab-equipment","tag-research-labs","tag-sterilizing-tips","tag-tips-and-tricks"],"acf":[],"yoast_head":"\nTop Tips for Autoclaving Pipette Tips - Priorclave<\/title>\n<meta name=\"description\" content=\"How to autoclave pipette tips without dampness: 1. Don\u2019t bother autoclaving sterile tips 2. 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