{"id":1266,"date":"2019-03-28T16:20:19","date_gmt":"2019-03-28T16:20:19","guid":{"rendered":"http:\/\/priorclavena.com\/?p=1266"},"modified":"2020-01-30T21:41:35","modified_gmt":"2020-01-30T21:41:35","slug":"red-bag-waste-autoclave-procedure","status":"publish","type":"post","link":"https:\/\/www.priorclave.com\/en-us\/2019\/03\/28\/red-bag-waste-autoclave-procedure\/","title":{"rendered":"Red-Bag Waste Autoclave Procedure"},"content":{"rendered":"<p class=\"p1\">It&#8217;s a dirty job, but someone&#8217;s gotta do it.<span class=\"Apple-converted-space\">\u00a0 <\/span>Following this three-step autoclave procedure will result in reliable sterilization of &#8220;red bag&#8221; waste.<span class=\"Apple-converted-space\">\u00a0 <\/span>(Assuming that you aren&#8217;t running a BSL3 facility or disposing of animal carcasses.)<\/p>\n<h2 class=\"p1\">Red Bag Waste Autoclave Procedure<\/h2>\n<p class=\"p1\">First things first, the <b>DON&#8217;T<\/b>s:<\/p>\n<p>&nbsp;<\/p>\n<ul class=\"ul1\">\n<li class=\"li1\"><b>DON&#8217;T<\/b> toss loose tips and pipettes into your biohazard waste bag; they&#8217;ll puncture the bag and may injure techs<\/li>\n<li class=\"li1\"><b>DON&#8217;T<\/b> fill bags more than two-thirds<\/li>\n<li class=\"li1\"><b>DON&#8217;T<\/b> double bag (double-bagging substantially increases cycle times; your load won&#8217;t be sterilized after a standard waste cycle!)<\/li>\n<\/ul>\n<p class=\"p1\">Now the <b>DO<\/b>s:<\/p>\n<h3 class=\"p1\">STEP #1: PREP<\/h3>\n<ol class=\"ol1\">\n<li class=\"li1\"><b>Add some water to the bag<\/b>\u2014this helps generate steam in the bag throughout the load<\/li>\n<li class=\"li1\"><b>Twist the top of the bag closed<\/b> and tape it with autoclave tape<\/li>\n<li class=\"li1\"><b>Label and date<\/b> the waste bag<\/li>\n<\/ol>\n<h3 class=\"p1\">STEP #2: LOAD<\/h3>\n<ol class=\"ol1\">\n<li class=\"li1\"><b>Place the red bag in an autoclave pan<\/b>\u2014leaky waste bags are the <i>worst<\/i> mess, easily jamming drains, vents, and other plumbing<\/li>\n<li class=\"li1\"><b>Make sure there is air-space between bags<\/b>\u2014better air circulation makes for more reliable sterilization<\/li>\n<li class=\"li1\"><b>Load pans into the autoclave<\/b>, again being sure that you&#8217;re leaving room for air flow<\/li>\n<\/ol>\n<h3 class=\"p1\">STEP #3: RUN<\/h3>\n<ol class=\"ol1\">\n<li class=\"li1\"><b>Check that the drain is clear<\/b>\u2014autoclaves used by several departments or for many waste loads often get clogged<\/li>\n<li class=\"li1\"><b>Tag the load with an <a href=\"https:\/\/www.3m.com\/3M\/en_US\/company-us\/all-3m-products\/~\/All-3M-Products\/Health-Care\/Medical\/Sterilization-Monitoring\/Chemical-Indicators\/?N=5002385+8707795+8707798+8711017+8711099+8753018+3294857497&amp;rt=r3\">integrator strip<\/a><\/b> to confirm sterilization<\/li>\n<li class=\"li1\"><b>Run your standard waste cycle<\/b><span class=\"s1\">\u2014<\/span>These vary by lab and application; make sure to use the settings approved in your lab<\/li>\n<li class=\"li1\"><b>Let the load cool<\/b><\/li>\n<li class=\"li1\"><b>Check the integrator strip<\/b>\n<ul class=\"ul2\">\n<li class=\"li1\">If it says <b>ACCEPT<\/b>, dispose of the sterilized bags according to your lab policy (likely in a hard-sided roll away &#8220;red bin&#8221;)<\/li>\n<li class=\"li1\">If it says <b>REJECT<\/b>, slap a new strip on it and run it again<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>It&#8217;s a dirty job, but someone&#8217;s gotta do it.\u00a0 Following this three-step autoclave procedure will result in reliable sterilization of &#8220;red bag&#8221; waste.\u00a0 (Assuming that you aren&#8217;t running a BSL3 [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":2859,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[73],"tags":[24,357,355,63,314,365,74,378,68,108,171,23,31],"class_list":["post-1266","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lab-autoclaves","tag-autoclave","tag-autoclave-procedure","tag-best-practices","tag-high-efficiency-research-autoclave","tag-lab-autoclaves","tag-lab-best-practices","tag-lab-practices","tag-lab-procedure","tag-lab-safety","tag-pathogenic-hazards","tag-pathogens","tag-research-grade-autoclave","tag-safety"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - 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