{"id":1267,"date":"2019-04-11T16:26:34","date_gmt":"2019-04-11T16:26:34","guid":{"rendered":"http:\/\/priorclavena.com\/?p=1267"},"modified":"2020-01-30T21:41:23","modified_gmt":"2020-01-30T21:41:23","slug":"autoclave-procedure-for-pipette-tips","status":"publish","type":"post","link":"https:\/\/www.priorclave.com\/en-us\/2019\/04\/11\/autoclave-procedure-for-pipette-tips\/","title":{"rendered":"Autoclave Procedure for Pipette Tips"},"content":{"rendered":"

The autoclave procedure for pipette tips is a little more involved than other red bag waste<\/a>.<\/p>\n

Why?<\/p>\n

Because pipette tips are pokey: They like to poke through bags and into people.\u00a0 <\/span>Even if your pipette tips themselves aren’t contaminated, they are a super-duper good vector for dangerous pathogens.\u00a0 <\/span>They’re also great at making sure goop from inside your red bags makes it out into your autoclave chamber, where they can gum up the plumbing.\u00a0 <\/span>And, if that wasn’t enough, the tips themselves are pretty adept at jamming drains and vents.<\/p>\n

Proper Pipette Tip Autoclave Procedure<\/h2>\n
    \n
  1. Line any old cardboard box with a red waste bag (PRO TIP<\/i>: Use a box that’s held together with glue or strips of autoclave tape; packing tape usually comes apart in a steam autoclave)<\/li>\n
  2. Toss used tips into the bag-lined box<\/li>\n
  3. When full, place the ENTIRE box into a fresh red waste bag<\/li>\n
  4. Twist and seal the inner<\/i> red bag (leave the outer<\/i> bag untied)<\/li>\n
  5. Label and date the outside<\/i> of the outer<\/i> bag with autoclave tape<\/li>\n
  6. Slap an integrator strip<\/a> on the outer bag<\/li>\n
  7. Place this bagged waste into a pan (as in the standard red-bag waste autoclave procedure<\/a>) and run it through a standard waste cycle (NOTE<\/b>: This varies by lab and application)<\/li>\n
  8. When the load is done, check the strip; if it says ACCEPT<\/b>, carry on\u2014if not, then run that load again<\/li>\n
  9. Let the load cool, then twist and tie off the outer<\/i> red-bag, place the whole mess in a secondary cardboard box, and dispose of that according to your lab’s procedure<\/li>\n<\/ol>\n

    Small-Lab Pipette Tip Autoclave Procedure<\/h2>\n

    If you don’t have a ton of pipette tips to destroy, consider this “lean” pipette-tip autoclave procedure:<\/p>\n

      \n
    1. Place pipettes in a standard pipette reservoir (aka a “pipette boat”) or biohazard pipette keeper box<\/li>\n
    2. Tape it shut with autoclave tape<\/li>\n
    3. Bag it in a red bag<\/li>\n
    4. Twist it, tape it, label and date it<\/li>\n
    5. Place in bin or tray<\/li>\n
    6. Slap an integrator strip<\/a> on it<\/li>\n
    7. Run it!<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

      The autoclave procedure for pipette tips is a little more involved than other red bag waste. Why? Because pipette tips are pokey: They like to poke through bags and into […]<\/p>\n","protected":false},"author":13,"featured_media":2862,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[73],"tags":[357,355,286,137,138,365,378,68,108,171,23,122],"class_list":["post-1267","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lab-autoclaves","tag-autoclave-procedure","tag-best-practices","tag-biomedical-research","tag-biosafety","tag-culture-of-safety","tag-lab-best-practices","tag-lab-procedure","tag-lab-safety","tag-pathogenic-hazards","tag-pathogens","tag-research-grade-autoclave","tag-research-grade-autoclaves"],"acf":[],"yoast_head":"\nAutoclave Procedure for Pipette Tips<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.priorclave.com\/en-us\/2019\/04\/11\/autoclave-procedure-for-pipette-tips\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Autoclave Procedure for Pipette Tips\" \/>\n<meta property=\"og:description\" content=\"The autoclave procedure for pipette tips is a little more involved than other red bag waste. 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