{"id":3170,"date":"2021-12-27T18:21:21","date_gmt":"2021-12-27T18:21:21","guid":{"rendered":"https:\/\/www.priorclave.com\/en-us\/?p=3170"},"modified":"2021-12-02T18:32:11","modified_gmt":"2021-12-02T18:32:11","slug":"how-to-autoclave-pipette-tips-quickly-racking-tips-for-sterilization","status":"publish","type":"post","link":"https:\/\/www.priorclave.com\/en-us\/2021\/12\/27\/how-to-autoclave-pipette-tips-quickly-racking-tips-for-sterilization\/","title":{"rendered":"How to Autoclave Pipette Tips: Quickly Racking Tips for Sterilization"},"content":{"rendered":"<p><span style=\"font-weight: 400\">At one time, many labs considered autoclaving and reusing plastic pipette tips an enormous waste of time. But with the recent revelation that <\/span><a href=\"https:\/\/www.priorclave.com\/en-us\/2021\/12\/13\/biotech-and-pharma-need-more-green-labs\"><span style=\"font-weight: 400\">only 4% of the world\u2019s largest publicly-traded biotech and pharmaceutical research labs have made even minimal climate commitments<\/span><\/a><span style=\"font-weight: 400\">, many labs are taking a fresh look at their lab practices. This increased interest in reducing single-use plastic waste\u2014coupled with <\/span><a href=\"http:\/\/www.priorclave.com\/en-us\/2021\/10\/29\/supply-chain-problems-renew-interest-in-pipette-tips-sterilization\/\"><span style=\"font-weight: 400\">ongoing supply chain issues<\/span><\/a><span style=\"font-weight: 400\"> delaying pipette tip deliveries\u2014has breathed new life into an age-old question:\u00a0<\/span><\/p>\n<p><strong>Does anyone know how to autoclave pipette tips efficiently? Is there <em>any<\/em> decent way to load loose pipette tips into tip boxes prior to autoclaving?\u00a0<\/strong><\/p>\n<h2><span style=\"font-weight: 400\">Tips for Racking Pipette Tips<\/span><\/h2>\n<p><span style=\"font-weight: 400\">Several manual tip racking hacks have been floating around for years. These include:<img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-2894 alignright\" src=\"https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-225x300.jpg\" alt=\"plastic pipette tips pose unique challenges\" width=\"225\" height=\"300\" srcset=\"https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-225x300.jpg 225w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-768x1024.jpg 768w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-1152x1536.jpg 1152w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-1536x2048.jpg 1536w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-64x85.jpg 64w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-128x171.jpg 128w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-240x320.jpg 240w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-320x427.jpg 320w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-360x480.jpg 360w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-375x500.jpg 375w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-480x640.jpg 480w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-540x720.jpg 540w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-640x853.jpg 640w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-720x960.jpg 720w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-800x1067.jpg 800w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-960x1280.jpg 960w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-1024x1365.jpg 1024w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-1280x1707.jpg 1280w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-1366x1821.jpg 1366w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-1600x2133.jpg 1600w, https:\/\/www.priorclave.com\/en-us\/wp-content\/uploads\/sites\/3\/2020\/01\/pipette-tips-autoclave-tape-WHAT-1920x2560.jpg 1920w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/span><\/p>\n<h3><span style=\"font-weight: 400\">Working in Quads<\/span><\/h3>\n<p><span style=\"font-weight: 400\">Start by gloving up. Hold a loose handful of tips in your cupped palm. Grab a clutch of four that are pointed in the same direction and rack them into a quadrant of the box. Grab another four and repeat. (With some tip sizes a clutch of three tips works better, but the fundamental concept is the same.)\u00a0\u00a0<\/span><\/p>\n<h3><span style=\"font-weight: 400\">Column Sorting<\/span><\/h3>\n<p><span style=\"font-weight: 400\">Alternatively, glove up and grab a small handful at tips. Grip them so they form a column down the base of your fingers. Ignore the ones pointing up and quickly rack those pointing down with your free hand. Dump the remainder back into the bin. Then grab a new handful, align them down the base of your fingers, and repeat.<\/span><\/p>\n<h2><span style=\"font-weight: 400\">Speed-Loading Pipette Tip Boxes<\/span><\/h2>\n<p><span style=\"font-weight: 400\">As with any tedious task, the time is guaranteed to fly if you <\/span><a href=\"http:\/\/youtu.be\/2Pbywpi64Tg?t=35\"><span style=\"font-weight: 400\">make a game of it<\/span><\/a><span style=\"font-weight: 400\">!\u00a0\u00a0<\/span><\/p>\n<blockquote><p><i><span style=\"font-weight: 400\">If you finish the box off with the exact number of tips you grabbed, you get 10 points! Seriously has anyone else noticed how much this happens??? OR IS IT JUST ME *sigh [<\/span><\/i><a href=\"http:\/\/www.reddit.com\/r\/labrats\/comments\/2ttarc\/any_tricks_to_manually_refilling_pipette_tip\/co26w9s\/\"><i><span style=\"font-weight: 400\">source<\/span><\/i><\/a><i><span style=\"font-weight: 400\">]\u00a0<\/span><\/i><\/p><\/blockquote>\n<p><span style=\"font-weight: 400\">But however you slice it, it&#8217;s still a tedious manual task. (And one unlikely to ever justify the <\/span><a href=\"http:\/\/www.directindustry.com\/prod\/hektros\/product-233803-2368111.html\"><span style=\"font-weight: 400\">$37,000 cost of automating it<\/span><\/a><span style=\"font-weight: 400\">.)\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400\">Thankfully, the folks over at <\/span><a href=\"http:\/\/www.thingiverse.com\/easylabsolutions\/designs\"><span style=\"font-weight: 400\">easylabsolutions<\/span><\/a><span style=\"font-weight: 400\"> have developed an extremely simple machine for quickly sorting and racking pipette tips:<\/span><\/p>\n<p><span style=\"font-weight: 400\">If you have access to a 3D printer, you can make your own pipette tip sorter.\u00a0 Easylabsolutions has published the files for both the <\/span><a href=\"http:\/\/www.thingiverse.com\/thing:4256563\"><span style=\"font-weight: 400\">10 \u00b5L pipette tip<\/span><\/a><span style=\"font-weight: 400\"> and <\/span><a href=\"http:\/\/www.thingiverse.com\/thing:4268044\"><span style=\"font-weight: 400\">200 \u00b5L pipette tip<\/span><\/a><span style=\"font-weight: 400\"> models. There are also a couple good <\/span><a href=\"http:\/\/www.thingiverse.com\/thing:4256563\/remixes\"><span style=\"font-weight: 400\">remixes and expansions<\/span><\/a><span style=\"font-weight: 400\"> of this design available on Thingiverse. Our favorite is <\/span><a href=\"http:\/\/www.thingiverse.com\/thing:4752098\"><span style=\"font-weight: 400\">this upgraded version<\/span><\/a><span style=\"font-weight: 400\"> with interchangeable plates. The design files can be readily adapted to sorting a variety of tips from different manufacturers.<\/span><\/p>\n<p><span style=\"font-weight: 400\">Now that your tips are all boxed up, make sure you know <\/span><a href=\"http:\/\/www.priorclave.com\/en-us\/2018\/09\/06\/top-tips-for-autoclaving-pipette-tips\/\"><span style=\"font-weight: 400\">how to autoclave pipette tips<\/span><\/a><span style=\"font-weight: 400\"> with minimal hassle.\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>At one time, many labs considered autoclaving and reusing plastic pipette tips an enormous waste of time. But with the recent revelation that only 4% of the world\u2019s largest publicly-traded [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":1186,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[465],"tags":[485,486,484,76],"class_list":["post-3170","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lab-practices","tag-autoclave-best-practices","tag-lab-sterilization","tag-pipette-tip-sterilization","tag-pipette-tips"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>How to Autoclave Pipette Tips: Quickly Racking Tips for Sterilization<\/title>\n<meta name=\"description\" content=\"Wondering how to autoclave pipette tips? 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