{"id":3200,"date":"2022-03-14T16:21:55","date_gmt":"2022-03-14T16:21:55","guid":{"rendered":"https:\/\/www.priorclave.com\/en-us\/?p=3200"},"modified":"2022-03-04T16:22:49","modified_gmt":"2022-03-04T16:22:49","slug":"how-to-autoclave-agar-plates-and-reduce-single-use-lab-plastics","status":"publish","type":"post","link":"https:\/\/www.priorclave.com\/en-us\/2022\/03\/14\/how-to-autoclave-agar-plates-and-reduce-single-use-lab-plastics\/","title":{"rendered":"How to Autoclave Agar Plates and Reduce Single-Use Lab Plastics"},"content":{"rendered":"<p><span style=\"font-weight: 400\">Given the quickening pace of global climate change, an increasing number of lab managers are looking for <\/span><a href=\"http:\/\/www.priorclave.com\/en-us\/2020\/07\/02\/finding-single-use-plastics-alternatives-for-research-labs\/\"><span style=\"font-weight: 400\">single-use plastics alternatives<\/span><\/a><span style=\"font-weight: 400\"> (and <\/span><a href=\"http:\/\/www.priorclave.com\/en-us\/2018\/12\/27\/we-need-sustainable-labs-for-sustainable-research\/\"><span style=\"font-weight: 400\">it&#8217;s about time<\/span><\/a><span style=\"font-weight: 400\">!). Making the transition to glass <\/span><a href=\"https:\/\/en.wikipedia.org\/wiki\/Agar_plate\"><span style=\"font-weight: 400\">agar plates<\/span><\/a><span style=\"font-weight: 400\"> is an excellent opportunity. Using your existing <\/span><a href=\"http:\/\/www.priorclave.com\/en-us\/autoclave-customers\/autoclaves-scientists-researchers\/\"><span style=\"font-weight: 400\">research autoclave<\/span><\/a><span style=\"font-weight: 400\"> and a supply of pyrex media plates, any lab can substantially reduce its negative environmental impact.<\/span><\/p>\n<h2><span style=\"font-weight: 400\">Why Autoclave Agar Plates?<\/span><\/h2>\n<p><span style=\"font-weight: 400\">Elicia Preston is a research scientist and lab manager in the Genetics department at the University of Pennsylvania Perelman School of Medicine and a big proponent of sustainable lab practices. As she told <\/span><a href=\"http:\/\/www.labconscious.com\/green-lab-tips\/2019\/7\/11\/how-to-switch-to-glass-petri-dishes\"><span style=\"font-weight: 400\">Labconscious in 2019<\/span><\/a><span style=\"font-weight: 400\">, &#8220;Labs use huge amounts of energy and produce megatons of waste. As a biologist my fascination with the complex beauty of nature coupled with the irony of trampling nature, to better understand it, frustrates me. So I do what I can to lessen this negative impact of our work.&#8221;<\/span><\/p>\n<p><span style=\"font-weight: 400\">As an example, she noted that her lab alone consumes hundreds to thousands of single-use plastic plates per month. Producing one pound of polystyrene media plates (i.e., about 36 dishes) takes 11.28 kWh of electricity and 20.54 gals of water. Doing so creates only about 2 ounces of solid solid waste, but emits two-and-a-half pounds of CO2. And bear in mind that we&#8217;re just talking about the resources consumed and waste produced when manufacturing media plates. There&#8217;s then the additional energy and CO2 required to ship the plates overseas and then later dispose of them.\u00a0\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400\">Preston notes that her university purchases more than ~400,000 disposable agar plates and petri dishes per year from just a single distributor. \u201cThis petri dish waste, if lined up, would fill the length of the state of Pennsylvania. In contrast, glass culture dishes are only be manufactured and shipped once, and would only enter landfills when broken.\u201d<\/span><\/p>\n<p><span style=\"font-weight: 400\">Preston discusses her approach to <a href=\"https:\/\/www.youtube.com\/watch?v=635lzgRkNuQ\">sustainable labs and glass media plates<\/a> here:\u00a0<\/span><\/p>\n<p><iframe loading=\"lazy\" title=\"Reusable Glass Petri Instructional\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/635lzgRkNuQ?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/p>\n<h2><span style=\"font-weight: 400\">How to Autoclave Agar Plates in your Lab<\/span><\/h2>\n<p><span style=\"font-weight: 400\">Ready to start the shift to reusable glass agar\/media plates? Here&#8217;s a proven workflow for sterilizing glass plates:<\/span><\/p>\n<ol>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Collect your glass plates in separate bins in your red bag waste area; once you have a full load of dishes, proceed to step #2<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Dump\/scrape the plates (this refuse should be <\/span><a href=\"http:\/\/www.priorclave.com\/en-us\/2019\/03\/28\/red-bag-waste-autoclave-procedure\/\"><span style=\"font-weight: 400\">processed as red bag waste<\/span><\/a><span style=\"font-weight: 400\">)<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Soak plates overnight in 5% bleach solution<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Use a scrubby sponge to wipe off any writing<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Sort your plates and rinse them off (it&#8217;s bad to expose the autoclave chamber to bleach)<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Load them into a perforated autoclave tray, being sure to leave space for good steam flow (using an autoclave pouch rack can help ensure that the steam contacts every surface) <\/span><a href=\"http:\/\/www.priorclave.com\/en-us\/2018\/11\/29\/lab-autoclave-procedure-the-futility-of-foil\/\"><span style=\"font-weight: 400\">DO NOT WRAP THEM IN FOIL<\/span><\/a><span style=\"font-weight: 400\">!<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Autoclave them for 30 minutes at 121\u00baC\u00a0<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Reuse!<\/span><\/li>\n<\/ol>\n<p><span style=\"font-weight: 400\">&#8220;It may seem like a lot of work,&#8221; Preston notes, &#8220;but it&#8217;s really not that bad. You just integrate it in [to your week and workflow] while you&#8217;re waiting for an incubation or a centrifuge [step]. It&#8217;s a good break when you need to not think so hard for a change.&#8221;<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Given the quickening pace of global climate change, an increasing number of lab managers are looking for single-use plastics alternatives (and it&#8217;s about time!). Making the transition to glass agar [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":2581,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[465],"tags":[490,456,492,491,196],"class_list":["post-3200","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lab-practices","tag-agar-plates","tag-culture-media-sterilization","tag-labware","tag-petri-dishes","tag-sustainable-labs"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>How to Autoclave Agar Plates and Reduce Single-Use Lab Plastics<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.priorclave.com\/en-us\/2022\/03\/14\/how-to-autoclave-agar-plates-and-reduce-single-use-lab-plastics\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"How to Autoclave Agar Plates and Reduce Single-Use Lab Plastics\" \/>\n<meta property=\"og:description\" content=\"Given the quickening pace of global climate change, an increasing number of lab managers are looking for single-use plastics alternatives (and it&#8217;s about time!). 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