{"id":3153,"date":"2022-10-21T03:14:21","date_gmt":"2022-10-21T03:14:21","guid":{"rendered":"https:\/\/www.priorclave.com\/en\/?p=3153"},"modified":"2022-10-27T00:59:41","modified_gmt":"2022-10-27T00:59:41","slug":"gravity-cycle-vs-vacuum-cycle-when-to-use-a-vacuum-cycle","status":"publish","type":"post","link":"https:\/\/www.priorclave.com\/en\/2022\/10\/21\/gravity-cycle-vs-vacuum-cycle-when-to-use-a-vacuum-cycle\/","title":{"rendered":"Gravity Cycle vs. Vacuum Cycle: When to Use a Vacuum Cycle"},"content":{"rendered":"

A standard autoclave cycle is a <\/span>gravity cycle<\/b><\/a>\u2014so-called because, as steam rises to fill the sterilizer chamber, it displaces the cold air already in the chamber, which is then drawn out of the vessel by gravity. Under many circumstances, this process works just fine\u2014and its simplicity ensures relatively minimal parts, electricity use, and cost.\u00a0<\/span><\/p>\n

However, sometimes a gravity cycle will be suboptimal; in those instances, a <\/span>vacuum cycle<\/b> works better. A vacuum cycle doesn\u2019t wait for gravity to do the work. Instead it forces cold pockets out of the chamber and ensures even steam contact throughout the load. Adding just one pre-cycle vacuum pulse improves sterilization by about 90%; using three pre-cycle vacuum pulses virtually guarantees successful sterilization.\u00a0<\/span><\/p>\n

A \u201cpre-vac\u201d vacuum cycle can make a significant difference under many circumstances. But there are several key situations when you\u2019re sure to benefit.<\/span><\/p>\n

Five Times to Run a Vacuum Instead of Gravity Cycle<\/span><\/h2>\n
    \n
  1. Porous loads <\/b><\/a>are a challenge to fully permeate with steam without a vacuum cycle, leaving cold pockets that don\u2019t get sterilized.\u00a0<\/span><\/li>\n
  2. Erlenmeyer flasks, tubing, and other <\/span>narrow-mouthed containers <\/b>can trap cold air and sometimes prevent complete sterilization. An autoclave that allows for \u201cpre-vac\u201d cycles can help with this. An autoclave with a vacuum pump allows you to draw a partial vacuum in the chamber during the initial heating period when the chamber fills with steam. \u201cPulsing\u201d this vacuum entirely removes pockets of cold air from the chamber that might otherwise linger. The vacuum improves reliability of sterilizing every load, every time.<\/span><\/li>\n
  3. Because vacuum cycles speed heating and improve consistency throughout the chamber, they can be helpful when it\u2019s important to run a non-liquid<\/a> load<\/span> as quickly as possible. <\/b>Rather than wait passively for gravity to pull cold air out, the vacuum cycle speeds the process by actively pumping cold air out of the chamber.\u00a0<\/span><\/li>\n
  4. A vacuum pump also permits for a \u201cpost-cycle vacuum\u201d stage, which enables your load to <\/span>finish dryer <\/b>(even when faced with unfortunate\u2014though all too common\u2014lab practices, like <\/span>foiling beakers<\/span>)<\/span>.<\/b><\/li>\n
  5. Vacuum cycles benefit <\/span>waste loads<\/b> as well, ensuring that plastic especially\u2014which might otherwise trap cold air, preventing complete sterilization\u2014heats consistently and thoroughly enough to complete decontamination prior to disposal.<\/span><\/li>\n<\/ol>\n

    Have you been relying on gravity to do a vacuum\u2019s job? Priorclave builds your autoclave to order, so we build it according to your lab\u2019s unique requirements and demands. <\/span>Get in touch<\/span><\/a> with us to discuss what combination of features will serve you best.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

    A standard autoclave cycle is a gravity cycle\u2014so-called because, as steam rises to fill the sterilizer chamber, it displaces the cold air already in the chamber, which is then drawn […]<\/p>\n","protected":false},"author":17,"featured_media":3154,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[95,86],"tags":[306,96,364],"class_list":["post-3153","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lab-autoclaves","category-steam-autoclaves","tag-gravity-cycle","tag-lab-practices","tag-vacuum-cycle"],"acf":[],"yoast_head":"\nGravity Cycle vs. Vacuum Cycle: When to Use a Vacuum Cycle<\/title>\n<meta name=\"description\" content=\"A standard "gravity cycle" is usually good enough. But some sterilization tasks call for something more. 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